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Surveillance of leptospirosis and analysis of Leptospira serovarsin Fujian province, China, in 2012

XU Guo-ying, LIN Dai-hua, PAN Min-nan, LI Shu-yang, WANG Jia-xiong, ZHOU Shu-heng,HE Shi, LIU Jing, HAN Teng-wei, CHEN Yang, DENG Yan-qin

Abstract （246）

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Objective To investigate Leptospira serovars in Fujian province, China, and to provide a scientific basis for the prevention and control of leptospirosis. Methods Five investigation sites were established according to the epidemic situation of this disease. In order to determine the main serovars of Leptospira carried by humans and host animals, Leptospira was cultured with blood, kidney, or urine collected from rats, pigs, and frogs. The level of anti-Leptospira antibody was determined in human and rat sera. Serovars and infection status of Leptospira were analyzed based on these studies. Results The investigation of hosts showed that the population density of rodents was 5.83% in the open air of the main epidemic area. Main species were Rattus tanezumi, R. losea, and Niviventer fulvescens, which accounted for 29.87%, 28.19%, and 17.11%, respectively. Leptospira was first isolated from N. coxingi in Changtai county. The positive rate of serum antibody in rats was 11.04%. Autumnalis, Australis, and Grippotyphosa were three of the dominant serotypes in rats. The detection rate of serum antibody in humans was 8.25%. Autumnalis, Pyrogenes, Hebdomadis, and Australis were dominant serotypes in humans. Conclusion The serovars of Leptospira carried by rats and humans are complex in Fujian, which means leptospirosis surveillance should be well performed.

2014, 25 (5): 456-458. doi: 10.11853/j.issn.1003.4692.2014.05.019

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Epidemiological surveillance of hemorrhagic fever with renal syndromeand associated reservoir hosts during 2012 in Fujian province, China

CHEN Yang, LIN Daihua, CHEN Liang, LIN Wen, WANG Jiaxiong, HAN Tengwei, NIU Jing, LI Shuyang, HE Shi, DENG Yanqin

Abstract （377）

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Objective To elucidate the epidemiological characteristics of hemorrhagic fever with renal syndrome (HFRS) and associated reservoir hosts in Fujian province, China and to provide a scientific basis for the prevention and control of this disease. Methods The data on the epidemic situation of HFRS in Fujian province were subjected to statistical analysis. Rodents were captured using cages at night, and the population density and species composition of captured rodents were determined. Hantavirus antigen was detected and identified from rodent lungs by indirect immunofluorescence assay. Results A total of 373 cases of HFRS were reported in Fujian in 2012, with an incidence of 1.0027/100 000. The cases distributed in 61 counties, county?level cities, or districts, particularly in cities of Nanping, Fuzhou, Quanzhou, and Ningde. The incidence of HFRS was the highest in counties of Songxi, Zhouning, Shaowu, and Zhenghe. The surveillance of rodent populations showed that the mean trap catch rate was 5.32% indoors. The Norway rat (Rattus norvegicus), the reservoir of Soul virus, was the predominant species. In the mixed epidemic area, the mean trap catch rate of rodents was 3.62% in the field, and R. fulvescens was the predominant species. HFRS virus antigen was detected for the first time from R. norvegicus and R. tanezumi in county of Jiangle and R. norvegicus in county of Wuping. Conclusion R. norvegicus is still the main reservoir host of HFRS in Fujian. In 2012, the incidence of HFRS in Fujian increased significantly, especially in counties of Songxi county and Zhouning. Integrated rodent management should be implemented, and HFRS bivalent vaccine should be widely administered among the high risk populations, so as to reduce the incidence and prevent the outbreak of HFRS.

2014, 25 (2): 177-179. doi: 10.11853/j.issn.1003.4692.2014.02.025

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Epidemiological investigation of hemorrhagic fever with renal syndrome in Fujian province, China in 2011

CHEN Yang, HE Shi, LIN Wen, LI Shu-yang, CHEN Liang, DENG Yan-qin

Abstract （395）

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Objective To investigate the epidemic situation of hemorrhagic fever with renal syndrome (HFRS) in Fujian province, China and to provide a scientific basis for the prevention and control of this disease. Methods The data on the epidemic situation of HFRS in Fujian province were subjected to statistical analysis. Rodents were captured using cages at night. The density of captured rodents was calculated, and their species were identified. Hantavirus antigen was detected and identified from rodent lungs by indirect immunofluorescence assay. Results A total of 259 cases of HFRS were reported in Fujian in 2011, with an incidence of 0.70/105. The cases were distributed in 64 counties, county-level cities, or districts, particularly in Nanping, Ningde, Fuzhou, and Quanzhou. The incidence of HFRS was the highest in Songxi county and Zhouning county. The surveillance of rodents showed that the mean density of rodents was 8.22% indoors, and Rattus norvegicus, which carried Soul virus (typeⅡ), was the dominant species. In the mixed epidemic area, the mean density of rodents was 6.53% in the field, and R. fulvescens was the dominant species, followed by Apodemus agrarius, which carried Hantaan virus (type Ⅰ). HFRS virus antigen was first detected from R. norvegicus in Dehua county. Conclusion R. norvegicus is still the main host animal of HFRS in Fujian, and A. agrarius is the main host of HFRS in the field of mixed epidemic area in the northeastern area of Fujian. In 2011, the incidence of HFRS in Fujian increased significantly, especially in Songxi county and Zhouning county. Rodent control should be well performed, and HFRS bivalent vaccine should be widely used among the high-risk population, so as to reduce the incidence and prevent the outbreak of HFRS.

2013, 24 (4): 361-363. doi: 10.11853/j.issn.1003.4692.2013.04.026

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Surveillance and isolation virus of hemorrhagic fever with renal syndrome at different epidemic areas in Fujian

CHEN Yang, HE Shi, LI Shi-Qing, LIN Dai-Hua, LI Shu-Yang, ZHOU Shu-Heng, CHEN Liang, WANG Ling-Lan

Abstract （1248）

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【Abstract】 Objective To understand the pattern of epidemic areas of hemorrhagic fever with renal syndrome (HFRS) in Fujian and to provide scientific basis for its prevention and control. Methods Rats were captured in springs and autumns by live snap method, and its density and specie composition were investigated. The virus antigen from rodent lungs was isolated and identified. Results The dominant species in residential areas was Rattus norvegicus in Rattus type areas and the mixed?type area, which density were 4.91% and 5.73% at home respectively. While in the field, it was Apodemus agrarius with the average density of 12.95%, which was obviously higher in autumn and winter than that in spring. R.norvegicus mainly carried with Type Ⅱ virus, and Ap.agrarius with Type Ⅰvirus. A44 and R50 virus strain were isolated from them, respectively. Conclusion R.norvegicus was still the main host of HFRS in Fujian. Ap.agrarius was the main host in the field in the diversity epidemic area. It should strengthen the surveillance and the control of HFRS in main epidemic areas.

2009, 20 (4): 352-354.

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Application of the double antigens sandwich ELISA in serodiagnosis of hemorrhagic fever with renal syndrome

WU Shou-li; HE Shi; LI Shi-qing; LIN Dai-hua; LI Shu-yang; GHEN Liang; YAN Yan-sheng

Abstract （1172）

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Objective To establish a sensitive and specific method for detecting total antibodies in sera of hemorrhagic fever with renal syndrome(HFRS) patients and rodents by the double antigens sandwich ELISA. Methods The double antigens sandwich ELISA was established with coating antigen e1.3S from Hantavirus (HV), combining with recombinant antigen e6-119 labeled with HRP. The method was used to detect total antibodies in sera of HFRS patients and rodents, and the results were compared to that of indirect fluorescent assay(IFA). Results Five hundred and sixty-six samples (188 human sera and 378 rodents sera) were detected by using the double antigens sandwich ELISA and IFA. The total coincidence rate of the results by the two methods was 97.70%. Compared with IFA, the ELISA for the detection of total antibodies showed 97.54% in sensitivity and 97.87% in specificity. Conclusion The double antigens sandwich ELISA showed higher sensitivity and specificity for the detection of total antibodies, which can be applied in the large-scale epidemiologic survey.

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Observation on the Effect of Cyhalothrin Against the Dissociated Flea on the Ground

LUO Qi-song*;HE Shi-yuan;TIAN Zong-mao;et al

Abstract （943）

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Objective To observe and evaluate the effect of cyhalothrin against flea.Methods To chose two natural villages as experimental units and one village as control unit.The change of flea index before and after the treatment was used to count the killing effect.The change of flea index of the control unit was used to rectify the effect of killing flea.Results The rate of killing flea in 0.03% experimental area was 94.66%.The rectified rate of killing flea was 94.21%.The rate of killing xenopsylla cheopis was 81.30%.The rectified rate of killing flea was 79.71%.The rate of killing flea with 0.05% was 100.00%.The rectified rate of killing flea was 100.00%.Conclusion The cyhalothrin had better effect against the flea in the plague endemic area of Dehong prefecture.

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Study on Vector of Orientia tsutsugamushi and Specimens of Blood Clots and Spleen Collected from Artificial Infected Mice and Field Rodents by Nested Polymerase Chain Reaction

Chen Liang; Yan Yansheng; He Shi

Abstract （926）

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Objective: To detect Orientia tsutsugamushi in Chigger Mites and the specimens collected from the rodents to analyse the epidemic trend of scrub typhus. To evalute the sensitivity and specificity of nested polymerase chain reaction and promote the research in molecular epidemiology of O.tsutsugamushi in the country. Methods: NPCR was used to detect O.tsutsugamushi DNA in Chigger Mites and samples collected from artificial infected mice and field rodents. Two pairs of primer were synthesized on the basis of the DNA sequence from Sta 58kDa group specific antigen gene. Product of PCR amplification was analysed by 2% agarose gel electrophoresis. PCR products showed positive for O.tsutsugamushi DNA were further identified by 12% PAGE and PGEM-3Zf(+)/Hae Ⅲ DNA was used as nucleotide molecular weight marker. The result indicated the band is corresponding to a 88bp DNA fragment from Sta 58 kDa gene of O.tsutsugamushi . Result: One of 29 (3.45 %) blood clots and one of 112 (0.98 %) spleen tissue from field rats were positive for 88bp DNA fragment. Eight DNA extracts from 39 (20.5%) chigger mites produced the predicted DNA fragment.The specimens of both blood clots and spleen tissues from the mice at day 3 of postinfection showed negative to the specific PCR product. But positive at day 6 and 9. Conclusion: NPCR has high sensitivity and specificity. Hence. The PCR assay is a useful tool to investigate the natural epidemic foci of scrub typhus.